Phospho-ampk substrate motif

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August undefined, 2024

WebNov 3, 2015 · To characterize the AMPK network, we conducted a screen to identify direct AMPK substrates and phosphorylation sites. We used an analog-specific (AS) method of kinase-substrate identification (Banko et al., 2011, Shah et al., 1997), which utilizes a mutant of AMPK that accepts N 6-(phenethyl) ATPγS, a bulky form of ATP introduced into cells … WebTo predict potential AMPK substrates, a sequence window of −5 to +4 positions around each phosphorylation site was scored with the AMPK motif analyzer 27. Putative AMPK phosphorylation sites ...

Phosphorylation of PLIN3 by AMPK promotes dispersion of

WebApr 25, 2008 · Comparing the optimal motif we identified from the peptide library screen to all known well-established in vivo substrates of AMPK shows excellent concordance (Figure 1 B).Each of these substrates contains not only the required basic residue in −3 or −4, and hydrophobic residues in −5 and +4, but they also exhibit strong bias toward the secondary … WebApr 11, 2024 · In LNCaP cells, inhibition of GSK3β by LiCl led to increased phosphorylation of its substrate glycogen synthase (pGS ... TPD52 characteristic D2-motif, PEST sequences, ... The results showed that the AICAR an activator of AMPK by phosphorylation significantly downregulated TPD52 expression in both LNCaP and VCaP cells. While investigating ... slowtwitch.com forum

AMPK-PERK axis represses oxidative metabolism and ... - Cell Reports

WebAnalysis of phospho-AMPK substrate motif antibody immunoprecipitates by MS/MS. (A, B) Primary hepatocytes were isolated from male C57BL/6 mice (12-week-old). Hepatocytes were incubated with ... WebMar 13, 2024 · The first, based on the AMPK phosphorylation site of ACC at Ser79, was the development of a specific peptide substrate (the SAMS peptide) that could be used to measure AMPK activity 336. WebAug 12, 2024 · The C-terminal domain contains multiple protein kinase A (PKA) phosphorylation sites and is highly conserved between SIK1 and SIK2. 22 Like other AMPK family members, an activation loop (T-loop ... slowtwitch.com

Motif affinity and mass spectrometry proteomic approach for the ...

WebMay 1, 2015 · Here, we report an affinity proteomicstrategy for the discovery and validation of AMPK targets using an antibody to isolate proteins containing the phospho-AMPK substrate recognition motif from hepatocytes that had been treated with pharmacological AMPK activators. WebFeb 16, 2024 · A previous quantitative phospho-proteomics study uncovered an overall comprehensive regulatory role of AMPK during DDR with multiple newly identified substrates (Chen et al., 2024), while the intact role and related mechanism of AMPK in DSBR remained unknown.TALEN (transcription activator-like effector nuclease) technology was … slow twist loose ring snaffle bitWebFeb 15, 2011 · To date, people have gathered large amounts of phosphorylation data, making it possible to perform substrate-driven motif discovery using data mining techniques. Results: We describe an algorithm called Motif-All that is able to efficiently identify all statistically significant motifs. The proposed method explores a support … slowtwitch classifieds

"WebAMP-activated protein kinase (AMPK) is a conserved energy sensor that plays roles in diverse biological processes via phosphorylating various substrates. Emerging studies … " - Phospho-ampk substrate motif

Phospho-ampk substrate motif

Global phosphoproteomic analysis reveals ARMC10 as an AMPK substrate …

WebNov 3, 2015 · We provide an AMPK motif matrix and a pipeline to predict additional AMPK substrates from quantitative phosphoproteomics datasets. As AMPK is emerging as a … WebPTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) Kit Description: PTMScan® Technology employs a propri-etary methodology from Cell Signaling Technology for …

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WebAMPK is a highly conserved sensor of intracellular adenosine nucleotide levels that is activated when even modest decreases in ATP production result in relative increases in … WebIP. Immunoprecipitation of extracts from H1650 cells using Phospho-AMPK Substrate Motif [LXRXX (pS/pT) MultiMab ™ Rabbit mAb mix. Lane 1 shows 10% input. Western blot analysis was performed using the same antibody. Western blot analysis of extracts from various mouse tissues using Phospho-AMPK Substrate Motif [LXRXX (pS/pT) MultiMab ...

WebFeb 6, 2024 · The phosphorylation of S461 in the presence or absence of cisplatin was measured by an antibody recognizing phospho-AMPK substrate motif [LXRXX(pS/pT)]. WebDec 3, 2024 · Introduction. 5' AMP-activated protein kinase (AMPK) family members share a high degree of sequence similarity within the N-terminal kinase domains [], but their regulatory C-termini are far more divergent.Phosphorylation of a conserved threonine residue within the activation loop is necessary for the activation of all AMPK family …

WebJan 4, 2024 · To search for AMPK-dependent phosphorylation events on PERK, we used a phospho-AMPK substrate motif antibody to IP proteins harboring an ... motif and investigated PERK on these IPs by immunoblotting (Figure 2C, left). In phospho-AMPK substrate IPs, we observed an increased amount of PERK in GSK621- compared with … WebJan 10, 2024 · We found that the phospho-AMPK substrate motif antibody, which is more specific than the phospho-Ser antibody, was able to detect MFF phosphorylation. However, the phospho-AMPK substrate... We would like to show you a description here but the site won’t allow us.

Web21 hours ago · AMPK has previously been shown to phosphorylate RabGAPs directly thereby inducing an intramolecular autoinhibition . In mammalian cells, the AMPK-mediated phosphorylation of Ser168 near the N-terminus of TBC1D17 reduces its GAP activity toward its substrate Rab5 . Due to the structural similarities between TBC1D17 and TBC-7, and …

WebPhospho-AMPK Substrate Motif [LXRXX(pS/pT) MultiMab™ Rabbit mAb mix preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The … soha work for usWebJan 10, 2024 · Further analysis using an AMPK consensus phosphorylation motif indicated that 32 of these sites are likely direct AMPK phosphorylation sites. We validated one uncharacterized protein, ARMC10, and demonstrated that the S45 site of ARMC10 can be phosphorylated by AMPK both in vitro and in vivo. soha websiteWebJul 9, 2024 · a p-AMPK substrate motif-specific antibody showing phosphorylation of GST-RUNX2-WT but not in lanes of serine-specific mutant of RUNX2-S118A, and GST control … slowtwitch.com dtoce cor scoreWebNov 14, 2024 · Here, we provide evidence that PLIN3 is a novel and direct substrate of AMPK and PLIN3 phosphorylation by AMPK promotes LD dispersion, which implicates PLIN3 in lipolysis pathways. PLIN3 and PLIN2 are less efficient than PLIN1 at inhibiting basal lipolysis (Patel et al., 2014; Sanders et al., 2015 ). sohawa universityWebWe systematically explored the effects of the AMPK-MFN2 signaling axis on dynamic changes of mitochondria, autophagy and MAMs in response to energy stresses. Results Energy stress promotes MAM formation, autophagy and mitochondrial fission slow tv train rideWebDec 23, 2010 · Many in vivo substrates of AMPK not only conform to this motif but also bind to the phospho-binding protein 14-3-3 inducibly upon phosphorylation by AMPK. We therefore screened for proteins that bound to recombinant 14-3-3 in wild-type (WT) but not AMPK-deficient cells, and only under conditions of energy stress, when AMPK would be … slow twitch colorWebMar 18, 2024 · Although the phosphorylation motif is not a perfect fit, it has been previously shown that AMPK can potently phosphorylate its substrate without the canonical motif, such as ULK1 [Citation 63–65]. Importantly, while WT GST-TFEB is efficiently phosphorylated by AMPK in vitro , a truncation of the 36 C-terminal residues led to a dramatic ... sohay clothing