WebSep 18, 2024 · Summary. This protocol describes immunoprecipitation of proteins associated with FLAG-tagged recombinant proteins followed by mass spectrometry … WebFLAG-tag-based purification has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography . A FLAG-tag can be used in many different assays that require recognition by an antibody.
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WebRecombinant Full Length Human NCL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells. Description : Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. WebApr 13, 2024 · The no FLAG affinity purification was used as a control. d Western blot analysis with an anti-FLAG-tag antibody for the validation of the presence of MRPS17 … imt foundry
FLAG-Tag Purification
WebJul 31, 2024 · The FLAG-CFTR-His protein was eluted with 200 μg/mL FLAG ® peptide (DYKDDDDK, Sigma-Aldrich) in buffer A with 0.025% LMNG or buffer B with 0.025% amphipol using the magnetic rack after 45 min incubation with shaking at 4 °C. The purified protein at 500 μL was loaded on a Superose 6 Increase 10/300 column (GE Healthcare) … WebHepatitis B virus (HBV) core protein (HBc) plays many roles in the HBV life cycle, such as regulation of transcription, RNA encapsidation, reverse transcription, and viral release. To accomplish these functions, HBc interacts with many host proteins and undergoes different post-translational modifications (PTMs). One of the most common PTMs is … WebAffinity-tagged purification. In two-step affinity-tagged protein purification, a protein is first purified by affinity chromatography, then desalted. In some medium pressure chromatography systems, such as the NGC medium pressure chromatography systems, these two steps can be automated. imtf trading